**Researchers Discover New Way to See Inside Living Cells with LIfE**
**Breakthrough imaging technique allows scientists to visualize cellular structures in unprecedented detail.**
**Summary of Key Points**
* Researchers have developed a new imaging technique called LIfE (Light microscopy with Fourier Encoding) that allows them to see inside living cells with unprecedented detail.
* LIfE uses a combination of light microscopy and Fourier encoding to create images that are both high-resolution and three-dimensional.
* The technique has already been used to image a variety of cellular structures, including mitochondria, nuclei, and the cytoskeleton.
* LIfE has the potential to revolutionize the study of living cells and could lead to new discoveries about how cells function.
**Article Body**
Researchers at the University of California, Berkeley have developed a new imaging technique that allows them to see inside living cells with unprecedented detail. The technique, called LIfE (Light microscopy with Fourier Encoding), uses a combination of light microscopy and Fourier encoding to create images that are both high-resolution and three-dimensional.
Traditional light microscopy techniques are limited in their ability to image living cells because they can only capture two-dimensional images. This makes it difficult to see the intricate structures inside cells, which are often three-dimensional. LIfE overcomes this limitation by using Fourier encoding to capture three-dimensional information from the sample.
Fourier encoding is a mathematical technique that can be used to encode information into the frequency domain. This allows the researchers to capture more information from the sample than they could with traditional light microscopy techniques.
The researchers have already used LIfE to image a variety of cellular structures, including mitochondria, nuclei, and the cytoskeleton. They have also used LIfE to track the movement of proteins and other molecules inside cells.
LIfE has the potential to revolutionize the study of living cells. It could allow scientists to make new discoveries about how cells function and could lead to the development of new drugs and therapies.
**Additional Information**
* The research was published in the journal Nature Methods.
* The researchers have made the LIfE software available open source so that other scientists can use it.
* The researchers are currently working on developing LIfE for use in clinical settings..
